repp make features
Find or build a plasmid from its constituent features
repp make features "[feature],...[featureN]" [flags]
Examples
repp make features "BBa_R0062,BBa_B0034,BBa_C0040,BBa_B0010,BBa_B0012" --backbone pSB1C3 --enzymes "EcoRI,PstI" --dbs igem
Options
-b, --backbone string backbone to insert the fragments into. Can either be an entry
in one of the dbs or a file on the local filesystem.
-d, --dbs string comma separated list of sequence databases by name
-e, --enzymes string comma separated list of enzymes to linearize the backbone with.
The backbone must be specified. 'repp ls enzymes' prints a list of
recognized enzymes.
-x, --exclude string keywords for excluding fragments
-h, --help help for features
-p, --identity int %-identity threshold (see 'blastn -help') (default 98)
-o, --out string output file name
Options inherited from parent commands
-c, --config string path to a config file
-v, --verbose write DEBUG logs
SEE ALSO
- repp make - Make a plasmid from its expected sequence, features, or fragments