repp make fragments
Build a plasmid from its constituent fragments
Synopsis
Prepare a list of fragments for assembly via Gibson Assembly. Fragments are checked for existing homology with their neighbors and are prepared for assembly with PCR.
repp make fragments [flags]
Options
-b, --backbone string backbone to insert the fragments into. Can either be an entry
in one of the dbs or a file on the local filesystem.
-d, --dbs string comma separated list of sequence databases by name
-e, --enzymes string comma separated list of enzymes to linearize the backbone with.
The backbone must be specified. 'repp ls enzymes' prints a list of
recognized enzymes.
-h, --help help for fragments
-i, --in string input file name (FASTA or Genbank)
-o, --out string output file name
Options inherited from parent commands
-c, --config string path to a config file
-v, --verbose write DEBUG logs
SEE ALSO
- repp make - Make a plasmid from its expected sequence, features, or fragments